علوم زیستی دریا
Fatemeh Elmi; Sekineh Khazaee Pool; Maryam Mitra Elmi
Abstract
Mineralized collagen has diverse biological advantages, biocompatibility and biodegradability. In this study, the process of mineralization of the protein collagen type I extracted from the scales of white fish (Rutilus Frisii Kutum), was investigated in the Simulated Body Fluid (SBF). During immersion ...
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Mineralized collagen has diverse biological advantages, biocompatibility and biodegradability. In this study, the process of mineralization of the protein collagen type I extracted from the scales of white fish (Rutilus Frisii Kutum), was investigated in the Simulated Body Fluid (SBF). During immersion in SBF, tricalcium phosphate crystals were precipitated into collagen nanofibrils. SDS-PAGE electrophoresis confirmed the presence of type I collagen, including 3 spiral strands. Infrared Fourier Transform (FTIR) spectroscopy technique was used to characterize extracted collagen nanofibrils and mineralazied collagen. The presence of 60% of minerals in the mineralized Acid Soluble Collagen (ASC) was confirmed by TGA. DSC was used to determine the denaturation temperature of mineralized ASC at 147.5 °C, indicating a higher thermal stability of mineralized collagen. TEM images showed irregular and dense formation of tricalcium phosphate nanoparticles on collagen nanofibrils. Mineralized collagen protein can provide new insight into producing porous scaffolds for tissue engineering, dental implants, and providing medical equipment.